Improving control of Fusarium wilt of leguminous plants by combined application of biocontrol agents.

摘要

In dual culture tests, Trichoderma pseudokoningii and Bacillus subtilis parasitized and inhibited the growth of Fusarium oxysporum f. sp. fabae and of F. oxysporum f. sp. lupini, which cause wilt on broad bean (Vicia faba) cv. Giza 2 and lupin cv. Giza 2, respectively. When applied to the seeds of these crops in field experiments conducted in Egypt, both antagonists controlled wilt of broad bean and lupin (75.2% healthy plants). A mixture of both biological control agents was more effective than either agent used alone. Moreover, the biological control agents provided a higher percentage of healthy plants than a fungicide tested for comparison. Fresh filtrate of both antagonists was more effective in suppressing pathogen growth than stored filtrate..