Ren1~d and Ren2 cooperate to preserve homeostasis: evidence from mice expressing GFP in place of Ren1~d

摘要

To distinguish the contribution of Ren1~d and Ren2 to kidney development and blood pressure homeostasis, we placed green fluorescent protein (GFP) under control of the Ren1~d renin locus by homologous recombination in mice. Homozygous Ren1~d-GFP animals make GFP mRNA in place of Ren1~d mRNA in he kidney and maintain Ren2 synthesis in the juxtaglomerular (JG) cells. GFP expression provides an accurate marker of Ren1~d expression during development. Kidneys from homozygous animals are histologically normal, although with fewer secretory granules in the JG cells. Blood pressure and circulating renin are reduced in Ren1~d-GFP homozygotes. Acute administration of losartan decreases blood pressure further suggesting a role for Ren2 protein in blood pressure homeostasis. These studies demonstrate that, in the absence of Ren1~d, Ren2 preserves normal kidney development and prevents severe hypotension. Chronic losartan treatment results in compensation via recruitment of both Ren1~d- and Ren2-expressing cells along the preglomerular vessels. This response is achieved by metaplastic transformation of arteriolar smooth muscle cells, a major mechanism to control renin bioavailability and blood pressure homeostasis.