Changes of protective enzymes and accumulation of polyamines induced by boron deficiency in roots of rape seedlings

摘要

The effects of boron deficiency on the membrane permeability, the lipid peroxidation of membrane, the activities of the protective enzymes and the accumulation of polyamines in the roots of rape (Brassica napus L. cv Zhongyou 821) plants were examined using solution culture experiment. Compared to the 20 mmol a m~(-3) treatment, boron-deficient treatment (2 mmol B m~(-3)) decreased root dry weight and increased the rate of solute leakage and malondialdehyde (MDA) concentration in the roots of rape: Similar patterns of change were observed in the level of lipid peroxidation (MDA concentration) and the rate of solute leakage under boron deficiency. Results suggested that a significant alteration of membrane composition had occurred under B deficiency. The concentration of putrescine and the ratio of putrescine to spermidine + spermine in the roots of rape increased significantly, and the activities of superoxide dismutase and catalase decreased in the roots of rape during B deficiency. But peroxidase activity in root of B-deficient plant was higher than that of control plant.