Detection of genetic diversity among sugarcane (Saccharum sp.) genotypes using Random Amplified Polymorphic DNA markers.

摘要

A Polymerase Chain Reaction (PCR) based marker assay was employed to detect genetic diversity at molecular level among local sugarcane genotypes, which were difficult to discriminate otherwise on the basis of morphological and physiological characters. Ten sugarcane genotypes were studied using Random Amplified Polymorphic DNA (RAPD) technique. Thirty-eight decamer primers generated a total of 258 discrete markers ranging from 0.2 to 2.5 kilo base pairs. Polymorphism rate of the markers for the genotypes examined was 28.58%. Average genetic similarity amongst the genotypes was 78.96%. Sugarcane genotypes SPSG-26, CP77-400, CSSG-668, CSSG-676 and NSG-311 showed the highest genetic diversity. Therefore, a high contrast is expected when crossing these with any other progenitors. Another interesting observation made in this study was that RAPD technique could be used to detect somatic tissue culture-induced genetic variation. RAPD markers seemed to be an effective tool for germplasm identification and breeding..