Malate Dehydrogenase 1 Banding Patterns of the Soybean Yellow foliage Mutants

摘要

Both nuclear and cytoplasmic inherited yellow foliage mutants have been identified in soybean [Gtycine max (L.) Merr.] (Palmer and Kilen 1987). Among the yellow foliage mutants, five nuclear inherited mutants, Genetic Types T253, T317, T323, T324 andT325, were malate dehydrogenase (MDH) [EC 1.1.1.37] 1 null (Mdhl-n) (Amberger et al 1992, Hedges and Palmer 1992). T317 was a somaclonal variant generated from tissue culture (Amberger et al 1992). T323, T324 and T325 were isolated in the progeny rows ofw4-m mutable line (Hedges and Palmer 1992, Palmer et al 1989). T253 , a spontaneous mutant in T239 background, was recorded originally as a tan saddle (k2) and yellow foliage (y20) mutant (Palmer 1984). Subsequently, T253 was tested for MDH banding pattern and confirmed to be malate dehydrogenase null which was allelic to the Mdh1-n alleles in T317, T324 and T325 (Hedges and Palmer 1992). Thus, T253 is a triple mutant with tan saddle seed coat (k2), Mdh1-n, and yellow foliage (y20) phenotype. No recombination between the k2 and y20 loci was detected with T253 crossed with wild type (Palmer 1984). All the above five yellow foliage, malate dehydrogenase null mutants were allelic at y20 and Mdhl-n loci (Amberger et al 1992, Hedges and Palmer 1992). The yellow foliage (y20) and Mdh1 null phenotypes in T317, T323, T324 and T325 always were co-inherited as a single recessive nuclear gene. No confirmed crossovers between the y20 and the Mdh1-n loci have been identified (Amberger et al 1992, Hedges and Palmer 1992).