A Rapid FT-IR-Based Method for Monitoring Detergent Removal from Biological Samples

摘要

In life science research, detergents are primarily used in sample preparation to liberate cellular components through membrane disruption and to solubilize lipid-associated proteins. However, detergents can interfere with applications such as enzyme-linked immunosorbent assays, isoelectric focusing, nuclear magnetic resonance spectroscopy, and mass spectrometry, and therefore, often need to be removed before analysis. Here we report a novel infrared-based method that permits fast and impartial analysis of detergent removal from biological samples. Because this method is spectrally based and label-free, determination of protein concentration can be performed simultaneously. An additional benefit of this technique is the small volume (2 μL) required for analysis, a significant fact given the often precious nature of biological samples.