Phylogenetic studies of selected isolates of Potato virus Y (PVY) infecting vegetable crops in KwaZulu-Natal, Republic of South Africa

摘要

Phylogenetic relationships of Potato virus Y (PVY) isolates infecting vegetable crops in KwaZulu-Natal, Republic of South Africa, were investigated. A 1 067 bp amplicon covering part of the coat protein gene and the 3'non-translated region (NTR) of three PVY~0 isolates infecting tomato (Solarium lycopersicum L), one PVY~° isolate infecting pepper [Capsicum annuum L.) and one PVY~N Wilga isolate infecting potato (Solanum tuberosum L.) were amplified, cloned and sequenced. The 5' NTR, P1, HC-Pro and part of the P3 regions (2 559 bp) of a PVYN isolate infecting potato were amplified, cloned and sequenced. Sequence data were compared with sequences of PVY isolates from different geographical locations and subjected to phylogenetic analyses. The PVY~N isolate clustered with the European sublineage N and has five unique amino acid residues. The PVY~N Wilga isolate branched with the American PVY~0 isolate in the O lineage. All PVY~0 isolates infecting tomato and pepper were grouped in a new sublineage within the O lineage.