Effect of liming using a partial mixing technique on reductions in the seed cadmium levels for soybeans (Glycine max (L.) Merr.) under field conditions

摘要

To reduce the availability of soil cadmium (Cd) to soybeans (Glycine max (L.) Merr.), we employed a liming by partial mixing (PM) technique in two drained paddy fields on Gray Lowland soils, which had 0.1molL(-1) hydrochloric acid-extractable Cd concentrations as high as 1.08 and 1.40mgkg(-1). Among the different application methods tested, PM application (PM2) using a width of 20cm and a depth of 20cm was found to be most appropriate for reducing the seed Cd concentration and to obtain the optimum yield at Site A. Under PM2, a liming rate of 38% of that for broadcast incorporated into the surface 15cm layer (Bc) was suitable to reduce the seed Cd concentration at Site A, whereas the lime rate with PM2 was set at 50% of that for Bc (PM2-50) at Site B due to the higher availability of soil Cd. The root system was limited within the range of lime and fertilizer application for PM2 as well as PM2-50; thus, the lime and fertilizer were supplied successfully to the rooting zone. The soil pH value was lower under PM2 at Site A and PM2-50 at Site B compared with Bc, whereas the seed Cd concentration was lower for PM2 and PM2-50. This may be explained by the intensive uptake of calcium and magnesium with PM2 as well as PM2-50. The seed Cd concentration in the cultivar Ryuhou at the target pH of 6.5 was approximately 30% lower with PM2-50 than Bc at Site B. In addition, the average seed Cd concentrations in one cultivar and two lines, characterized by the lower Cd uptake with higher retention in roots and higher accumulation in leaves, were approximately 40% lower compared with Ryuhou. Thus, the combination of liming with PM2-50 at the target pH of 6.5 and a low-Cd cultivar (or lines) minimized the seed Cd concentration. The highest seed Cd concentration was found in the first year of soybean cultivation, which was considered to be caused by the release of Cd from organic nitrogen compounds during the nitrogen mineralization process.