A Novel Method to Synthesize Versatile Multiple-Branched DNA (MB-DNA) by Reversible Photochemical Ligation

摘要

DNA has potential use both as a generic and a genetic material.Branched DNA molecules have various uses in a signal-amplification technology and in several types of nanotechnolo-gy,such as DNA computing,DNA nanostructures from self-assembled branched units,DNA sensors,and nanoelectron-ic devices.The past 10 years have produced remarkable success in the construction of DNA nanoarchitectures.For example,one- and two-dimensional DNA lattices and dendrimer-like DNA have been constructed from a rich set of branched DNA.Various autonomous DNA walker devices,based on DNA cleavage and ligation of branched DNA by using various enzymes,have also been explored theoretically and experimentally.