Glass-Bead-Based Parallel Detection of DNA Using Composite Raman Labels

摘要

Biomolecule detection has become increasingly important in biomedical research and disease diagnosis.[1]-[8] In particular, microarrays are quite promising because they allow one to carry out many assays simultaneously with rapid readout provided by a variety of labeling strategies (flourophores, chemiluminescent entities, or nanoparticles).[1]-[4] Such arrays have been widely used in genomics and proteomics research.[5] In a typical DNA microarray format, glass substrates are spotted with appropriate capture cDNA (>200 nucleotides) or synthesized oligonucleotide strands (25-80 nucleotides).[6] Fluorophore-labeled target DNA strands are captured via hybridization to the arrayed capture strands. Since each DNA spot in the microarray has been positionally encoded, the results can be easily determined, typically, by using a computer-controlled laser scanner system.