Pt Nanoparticles Functionalized with Nucleic Acid Act as Catalytic Labels for the Chemiluminescent Detection of DNA and Proteins

摘要

The unique electronic, optical, and catalytic properties of metal or semiconductor nanoparticles (NPs) are employed to develop labels for the detection of biorecognition events.[1] For example, Au NPs functionalized with nucleic acid have been used as optical labels for the colorimetric[2] or surface-plasmon-resonance[3] detection of DNA. Similarly, the catalytic enlargement of nucleic acid modified Au NPs acting as labels for the hybridization of DNA was used for the analysis of DNA by conductivity measurements.[4] In other reports, the dissolution of Au or Ag NPs associated with DNA complexes, and the electrochemical analysis of the released ions was used to detect DNA.[5] Also, Au NPs functionalized with nucleic acid were used as weight labels for the microgravimetric analysis of DNA using quartz-crystal-microbalance experiments.[6] Semiconductor NPs modified by nucleic acid have been used as labels for the direct fluorescence detection of biorecognition events[7] or as activators of FRET emission.[8] Semiconductor NPs were also used to detect DNA by photoelectrochemical means.