Method of forming a stable complex with any polypeptide transcript of DNA encoding a translation product, the nucleic acid construct for use in the method, the complex formed by the method, as well as functionality that utilizes the method Screening of mRNA or DNA encoding the protein and the protein

摘要

A stable linkage between a genotype and a phenotype in a cell-free system was successfully achieved by using interaction between a RNA-binding protein and RNA, between a DNA-binding protein and DNA, or by using a protein that inactivates a ribosome. Furthermore, it was found that functional proteins could be selected by using these stable linkages.