Determination of C3-C10 Aliphatic Aldehydes Using PFBHA Derivatization and Solid Phase Microextraction(SPME).Application to the Analysis of Beer

摘要

In the present study the SPME on-fibre derivatization of C3-C10 aldehydes with the use of PFBHA is presented.Various procedures for adsorption of PFBHA on SPME fibre were studied.Finally,headspace adsorption mode at 20deg C was adopted.After saturation with PFBHA,the SPME fibre was exposed to aldehydes in the headspace,and extraction was performed for 20 min.This approach was compared to the in-solution derivatization with PFBHA and syringe injection of the solution of oximes.The former,however,occurred to be much more sensitive and allowed the aldehydes to be extracted from complex matrices.The developed method was applied to the analysis of C3-C10 aldehydes in beer.They were determined at the level:0.07-83.20 mug L~(-1),depending on the type of aldehyde and the brand of beer.