GC-MS Analysis of Acidic Components in Aqueous Extracts of Coniferous Trees' Needles

摘要

GC-MS method has been applied to the identification of acidic components in the needles of common spruce,Scots pine,eastern white pine,and Douglas fir.The investigated species were present in the aqueous phase after steam distillation of the essential oils.The analytes were subsequently extracted with diethyl ether and separated using capillary gas chroma-tography in the form of trimethylsilyl derivatives (TMS).The investigated samples were complex multicomponent mixtures containing aliphatic mono-and dicarboxylic acids,hydroxycarboxylic acids,aromatic and alkyl-aromatic acids,and hydroxyl derivatives of benzaldehyde and acetophenone.Distillation of water under low pressure and subsequent GC-MS analysis of the residue have shown that the fraction of organic compounds that was not extracted with ether,consists mainly of mono-and disaccharides.For the first time the values of retention indices for 25 TMS have been determined.