Study of catalase immobilized on a silicate matrix for non-aqueous biocatalysis

摘要

Catalytic activity of catalase(CAT)immobilized on a modified silicate matrix to mediate decomposition of meta-chloroperoxibenzoic acid(3-CPBA)in acetonitrile has been investigated by means of quantitative UV-spectrophotometry.Under the selected experimental conditions,the kinetic parameters:the apparent Michaelis constant(K_M),the apparent maximum rate of enzymatic reaction(V~(app)_(max)),the first order specific rate constants(k_(sp)), the energy of activation(E_a)and the pre-exponential factor of the Arrhenius equation(Z_0) were calculated.Conclusions regarding the rate-limiting step of the overall catalytic process were drawn from the calculated values of the Gibbs energy of activation DELTA G*,the enthalpy of activation DELTA H*,and the entropy of activation DELTA S*.