A nondigestible saccharide, fructooligosaccharide,increases the promotive effect of a flavonoid,-glucosyl-isoquercitrin, on glucagon-likepeptide 1 (GLP-1) secretion in rat intestineand enteroendocrine cells

摘要

This study conducted in vivo and in situ experiments with rats to investigate the glucagon-likepeptide-1 (GLP-1) secretion in response to oral or ileal administration of -glucosyl-isoquercitrin(20–40 mmol in 2 mL; Q3G), fructooligosaccharides (200 mmol in 2 mL; FOS) and Q3G +FOS. Direct effects on GLP-1-producingL-cells were also examined by an in vitro study using amurine enteroendocrine cell line, GLUTag. To evaluate the plasma GLP-1 level, blood samplesfrom jugular cannula for in vivo and portalcannula for in situ experiments were obtained beforeand after administration of Q3G, FOS, or Q3G + FOS. We found tendencies for increases buttransient stimulation of GLP-1 secretion by Q3G in in vivo and in situ experiments. AlthoughFOS alone did not have any effects, Q3G + FOS enhanced and prolonged high plasma GLP-1level in both experiments. In addition, application of Q3G on GLUTag cells stimulated GLP-1secretion while FOS enhanced the effect of Q3G. Our results suggest that Q3G + FOS pos-sess the potential for the management or prevention of Type 2 diabetes mellitus (T2DM) byenhancing and prolonging the GLP-1 secretion via direct stimulation of GLP-1 producingL-cell.