The Efficiency of Mesenchymal Cell Intracerebral Transplantation for Corrections of Cerebral Microcirculation Age-Related Alterations in Rats

摘要

Using television-based vital microscopy and immunohistochemical analysis, we have assessed the effect of syngeneic mesenchymal stem-cell (MSC) intracerebral transplantation on the brain cortex structure and microcirculation in the pia mater of old rats. Setting up the "open field" system, we studied the effect of MSC transplantation on the position-finding and discovery behavior of old animals. We found that the density of microvascular network of the pia mater increased by about 1.9-fold in MSC recipients compared to age-matched controls. The density of the arteriolar area of microvascular network of the pia mater approximately doubled. The reactivity of newly developed arterioles was nearly equal to that of native microvessels. The intracerebral transplantation procedure was itself traumatic for rat brain cortex, but it did not affect microcirculation in the contralateral hemisphere. Intracerebral transplantation of MSCs did not improve the locomotor behavior and emotional stage of old rats; neither did it increase their position-finding and discovery activity.