Introduction of exogenous wild soybean DNA into cultivated soybean and RAPD molecular verification

摘要

The exogenous total DNA of the wild high-protein soybean was transferred to cultivated soybean thiough the pollen tube channel and the genomic variation of the transformed progeny was detected by the method of RAPD (Random Amplified Polymorphic DNA).Distinguished variations were found in one of the 7 transformed plants of the first generation (D_1) of which the traits of fruition, outward appearance, leaf shape and flower colour were almost identical with those of the recipient parent; of which grain weight, seed coat colour and stem strength were situated between the two parents; and there were greatly more pods per plant and 12.5% higher content of protein in seeds than that of the recipient parent. All the properties have been invariably inherited for 3 generations. Through RAPD analysis of the genomes of the donor, the recipient and the transformed progeny (D_3) as a group, DNA polymorphisms were found in amplified products by 24 of 150 primers. The results prove that the exogenous DNA causedthe distinct variance of the genome. The authors infer that the homogeneous recombination of large exogenous DNA is the main cause for the variance.