ANTIOXIDANT ACTIVITY OF DIOSGENIN FROM Dioscorea

摘要

We aimed to infer and determine the antioxidant activity of diosgenin from Dioscorea composita by employing the established in vitro hydroxyl free radical system. In this paper, the direct hydrolysis-extraction of diosgenin under biphasic reaction conditions was applied with a 1.5% yield of diosgenin. The antioxidant activity of diosgenin was initially determined by the Fenton reaction-methylene blue spectrophotometric method. Results indicate that the hydroxyl radical scavenging activity of diosgenin was more than that of L-ascorbic acid when the concentration of the scavenger was higher than 2.5 x 10(-3) mmol/l.