Enhancement of antimicrobial and long-term biostability of the zinc-incorporated hydroxyapatite coated 316L stainless steel implant for biomedical application

摘要

Antimicrobial hydroxyapatite (HAp) nanoparticles with different concentrations (0, 3, and 6 mol%) of zinc were prepared by the ultrasonication process. The prepared nanoparticles and chitosan (CTS) composite were coated on 316L stainless steel implant by spin coating technique. The powder samples were characterised by particle size analyser, X-ray fluorescence, and X-ray diffraction studies. The morphology of the coating was investigated by scanning electron microscopy. The diameter of the particle size decreased with increase in the concentration of zinc in HAp structure. The structure of the coated implant was found to be uniform without any cracks and pores. Antimicrobial activity of the composites against Bacillus subtilis, Staphylococcus aureus, Klebsiella pneumonia, Salmonella typhi and Pseudomonas aeruginosa was analysed. The results showed that the increase in the concentration of zinc enhances the antimicrobial properties of 316L stainless steel implant. The stability of the implant in physiological environment was characterised by electrochemical impedance spectroscopy and polarisation analysis. The higher concentration of the ZnHAp/CTS composite shows higher corrosion resistance than that of the HAp/CTS-coated implant. This study shows that the coating provides corrosion resistance to the stainless steel substrate in simulated body fluid (SBF). The in vitro bioactivity study of the coated samples immersed in SBF solution confirms the formation of bone-like apatite layer on the surface of the implant. Thus, highly biocompatible ZnHAp/CTS-coated materials could be very useful in the long-term stability of the biomedical applications.