Callus induction and plant regeneration from immature embryos of indica rice varieties.

摘要

Callus initiation and plant regeneration was studied from immature embryos of three indica rice varieties (Heera, Pankaj and Basmati-370). MS medium supplemented with 100 mg adenine sulfate, 2 mg 2,4-D and 0.5 mg benzyladenine/litre was suitable forcallus initiation. Callus proliferation and shoot bud regeneration was achieved on MS medium with 100 mg adenine sulfate, 2.0 mg benzyladenine and 1.0 mg NAA/litre. For root initiation, differentiated calluses were transferred to MS medium containing 1.0 mg kinetin and 5.0 mg NAA/litre. Twelve-day-old embryos of the dwarf cultivar Heera and the semi-tall Pankaj and 18-day-old embryos of the late variety Basmati 370 were most responsive to in vitro culture. Regeneration was 20% for Heera and Pankaj and6.6% for Basmati-370. Response to in vitro culture condition was varietal specific.