Long-Distance Migration and Colonization of Transplanted Neural Stem Cells

摘要

A recent study reported remarkable survival and exuberant axon outgrowth from transplants of neural stem cells (NSCs) in a fibrin matrix with growth factors that were grafted into a complete spinal cord transection site in rats (Lu et al., 2012). As part of the NIH-supported replication project (Facilities of Research Excellence-Spinal Cord Injury), we repeated key parts of that study. Because this is a surgical intervention that may depend on skills that require extensive experience, we felt that the goals of the replication would be best served if the same surgeon performed the lesion surgeries and transplants. Accordingly, Dr. P. Lu traveled to UCI to perform the surgical procedures with aid of our staff. Also, because the cells could be considered to be a "product" that was obtained for use in much the same way as stem cells from a company, the cells were prepared at UCSD and were delivered to UCI on the day of the grafting procedure.