首页> 外文期刊>Cellular and molecular biology >Measurement of DNA damage induced by irradiation with gamma-rays from a TRIGA Mark II research reactor in human cells using Fast Micromethod.
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Measurement of DNA damage induced by irradiation with gamma-rays from a TRIGA Mark II research reactor in human cells using Fast Micromethod.

机译:使用Fast Micromethod测量人类细胞中TRIGA Mark II研究反应器的伽马射线辐照引起的DNA损伤。

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摘要

The Fast Micromethod is a novel quick and convenient microplate assay for determination of DNA single-strand breaks. This method measures the rate of unwinding of cellular DNA upon exposure to alkaline conditions using a fluorescent dye which preferentially binds to double-stranded DNA. Here we applied this method to determine the levels of DNA single-strand breaks in HeLa cells induced by y-irradiation deriving from fission isotopes and activation products at the TRIGA Mark II research reactor in Mainz. An increased strand scission factor (SSF) value, which is indicative for DNA damage, was found at doses of 1 Gy and higher. A similar increase in SSF value, which further increased in a dose-dependent manner, was found in human peripheral blood mononuclear cells after irradiation with 6 MV X-rays from a linear accelerator to give a total exposure of 0.5 to 10 Gy.
机译:快速微方法是一种新型的快速便捷的微孔板测定法,用于测定DNA单链断裂。该方法使用优先结合双链DNA的荧光染料测量暴露于碱性条件下细胞DNA的解链速率。在这里,我们应用这种方法来确定在美因茨的TRIGA Mark II研究反应堆中,由裂变同位素和活化产物引起的y辐射诱导的HeLa细胞中DNA单链断裂的水平。在1 Gy或更高剂量下,发现增加的链断裂因子(SSF)值指示DNA损伤。在用线性加速器照射6 MV X射线后,人外周血单核细胞中SSF值出现类似的增加,并以剂量​​依赖性方式进一步增加,总照射量为0.5至10 Gy。

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