The Effect of Salivary Gland Extract oiLucillia Sericata Maggots on the Human Dermal Fibroblasts Proliferation

摘要

Problem statement: The aim of study was to investigate the effect of Salivary Gland Extract (SGE) of Lucillia sericata maggots on Human Neonatal Fibroblasts (B-HNF-1) proliferation and migration within collagen/hyaluronan membrane. Chronic, non-healing wounds are major health care problem worldwide. Nowadays, Maggot Debridement Therapy (MDT) has attracted much attention due to its successfull application in a treatment of chronic wounds where conventional treatment has failed. Maggot Excretion/Secretion (ES) contains specific proteinases which are positivelly involved in the remodeling of Extracelallar Matrix (ECM) components. Approach: Immunohistochemical methods using primary monoclonal antibody against marker vimentin was employed for the visualization of fibroblasts cultured within collagen hyaluronan membrane. Results: We found that maggot SGE at concentration of 2.4 and 4.8 gL mL~(-1) significantly induced the migration of fibroblasts into the membrane after 5 and 10 days of cultivation. On the other hand, SGE at concentration of 7.2 gL mL~(-1) reduced the migration of fibroblasts. Conclusion: The obtained results suggest that medicinal maggots secretion may speed up the wound healing process through the stimulation of fibroblast migration.However, further detailed research is needed to fully elucidate the substance (s) responsible for fibrloblast stimulation and migration.