Characterization and molecular mapping of a soft glume mutant in diploid wheat (Triticum monococcum L.).

摘要

Diploid wheat T. monococcum L. is a model plant for wheat functional genomics. A soft glume mutant was identified during manual screening of EMS-treated M₂ progenies of a T. monococcum accession pau14087. The seeds in the mature spike of the mutant could be easily threshed manually. The soft glume mutant with high sterility, tapering and broader spikes had also tougher rachis than the wild type parent. Genetic analysis of crosses of the mutant with wild type indicated that the mutant was monogenic recessive. To map the soft glume mutant, a mapping population was developed by crossing the soft glume mutant with wild Triticum boeoticum acc. pau 5088, having tough glumes and hard threshing. The soft glume mutant was mapped between SSR markers Xgwm473 and Xbarc69 on 7AmL chromosome of T. monococcum, with a genetic distance of 1.8 cM and 8.3 cM, respectively. The soft glum mutant mapped on 7AmL, being distinct from a previously mapped soft glume mutant in wheat, has been designated as sog2. The work on fine mapping of sog2 gene is in progress.