Isolation of an Antifungal Pathogenesis-Related Protein from Naked Oat (Avena nuda) Seeds

摘要

A novel antifungal protein was isolated from naked oat (Avena nuda) seeds by acetone fractionation, (NH4)(2)SO4 precipitation, Q-Sepharose ion-exchange chromatography, chitin affinity chromatography, and Sephacryl S-200 gel filtration chromatography. The antifungal protein exhibited a molecular mass of 23,760, as measured by gel filtration and SDS-PAGE. assisted laser desorption ionization-time of flight MS analysis indithat the protein was homologous with a permatin precursor from A. The protein from naked oats exhibited antifungal activity against sp. SQ01 (half-maximal inhibitory concentration [IC50] = Trichoderma spp. (IC50 = 0.83 mu M), and Chaetomium sp. R01.