Molecular and morphological characterization of Ektaphelenchoides hunti sp. n. (Nematoda: Ektaphelenchinae) from northern Iran

摘要

Ektaphelenchoides hunti sp. n. is described and illustrated based on morphological and molecular characters. The new species is characterized by its body length of 711 -929 mum in the female, offset head region, three incisures in lateral field, totalstylet length of 16-23 mum, excretory pore in middle of the metacorpus to slightly posterior to metacorpus base, hemizonid at 95-116 mum distance from the anterior end and males with a body length of 572-809 mum, 12.5-17.5 mum long spicules with roundedcondylus, moderately developed short rostrum with blunt end and without a cucullus. The new species most closely resembles E. compsi but differs by having three incisures in lateral field vs obscure, excretory pore in middle of the metacorpus to slightly posterior to metacorpus base, 75-93 mum from anterior end vs posterior to the median bulb 91-113 mum from the anterior end, shorter spicules (12.5-17.5 vs 19-24 mum) and basic differences in shape of the posterior body (tail). The comparisons with other species of Ektaphelenchoides are also discussed. Molecular analyses were performed based on 793 bp partial ribosomal DNA large subunit D2D3 and showed E. hunti sp. n to be different but closest to E. compsi and E. spondylis.