Activity of glycogen catabolism enzymes in Contracaecum rudolphii (Anisakidae)

摘要

Extracts of Contracaecum rudolphii L3 and L4 larvae, females and males were assayed for carbohydrate content and activities of enzymes that decompose glycogen and disaccharides. The sugar content was about 6-8% of nematode wet weight. In terms of their content, the saccharides assayed could be arranged in the following order: glycogen, glucose and trehalose. All carbohydrate concentrations were greater in females than in males. C. rudolphii shows activity of enzymes that decompose glycogen via phosphorolysis and hydrolysis. A high activity of glycogen phosphorylase (2.89 mmol mg~(-1)) was recorded in the L3 larvae; the activity observed in the adults was lower by half. The amylase activities in all the forms of the parasite were relatively high andpractically the same (0.163-0.175 U mg~(-1) and 0.93-1.03 mumol mg~(-1) for а-amylase and glucoamylase, respectively). Among disaccharidases (maltase, saccharase, lactase), the greatest activity was shown by maltase. It was particularly high (2.67muumolmg~(-1)) in the L3. The saccharase activity in these larvae was twice that of the adults. Lactase had the lowest activity (0.26-0.49 mumol mg~(-1)).