Cotransformation of Aspen and Birch with Three T-DNA Regions from Two Different Replicons in One Agrobacterium tumefaciens train

摘要

The cointegration rate into the aspen and birch genomes of foreign genes from a binary vector and a disarmed Ti plasmid pCBE21 carried by the same Agrobacterium tumefaciens strain was studied. The cotransformation rate for the genes within the Ti plasmid varied from 30 to 100%; while the transformation rate for the gene from T_L region was twofold higher as compared with the T_R region. On the average, the gene transfer from all three T-DNAs was recorded in 10. 9% of the transgenic lines. For the vector pBI121, the cotransformation rates for the genes from both regions of pCBE21 T-DNA were higher as compared with the vector pGS. In addition, a concurrent transfer of the genes from the Ti plasmid T_L and T_R regions was recorded only after the transformation with the vector pBI121. These results can be used for constructing woody plants containing several genes.