Peroxide damage of hemoglobin by the fenton system

摘要

The extent of hemoglobin peroxidation under the action of mixtures of ferrous salts and hydrogen peroxide has been investigated. The rate of accumulation of carbonyl-containing products of the protein fragmentation, detected as 2,4-dinitrophenylhydrazones, is independent of the pH of the reaction medium and proportional to the concentrations of hydrogen peroxide, ferrous ion, and hemoglobin. A chain radical mechanism of the peroxide fragmentation of the polypeptide chains of hemoglobin has been proposed, involving reactions of the alkoxyl radical of hemoglobin with ferrous ion and of the carbon-centered radical of the protein with dissolved oxygen as rate-limiting steps. Chain termination is therewith effected through cross recombination of the above radicals.