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Obtinerea si caracterizarea de suporturi biocompatibile sub forma de microparticule si filme de chitosan-alginat cu ureaza imobilizata

机译:以固定化脲酶的微粒和壳聚糖-藻酸盐薄膜形式获得和表征生物相容性载体

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摘要

This study focuses on the preparation and characterization of the microparticles and films from chitosan,alginate and alginate/chitosan in relation to their application as supports for the urease immobilization.These enzymes are imobilized by using the following adsorbtion on solid supports methods:direct immersion of the support into enzymatic solutions;direct attachment of the carbodiimide derivate or sodium citrate activated supports.Carbodiimide activated supports have proved to be the most appropriate imobilization procedure.In this case the immobilization yields exceed 70%.
机译:本研究着重于壳聚糖,藻酸盐和藻酸盐/壳聚糖的微粒和薄膜的制备和表征及其在尿素酶固定化载体上的应用。这些酶通过以下在固相载体上的吸附方法固定化:直接浸入碳二亚胺衍生物或柠檬酸钠活化的载体直接连接碳二亚胺活化的载体被证明是最合适的固定方法,在这种情况下固定产率超过70%。

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