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A nano-scale probing system with a gold nano-dot array for measurement of a single biomolecular interaction force

机译:具有金纳米点阵列的纳米级探测系统,用于测量单个生物分子相互作用力

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摘要

A new nano-scale probing system was proposed and developed to measure and analyze the interaction force between biomolecules at the single molecular level. A probing instrument adopting the working principle of atomic force microscopy (AFM) was designed to measure a single biomolecular interaction force with a patterned gold nano-dot array and functionalized AFM probe. The constructed probing system was evaluated through measurement of the unbinding force between a protein (Vibrio cholerae toxin B subunit) and a carbohydrate (GM1 pentasaccharide) as a model single biomolecular interaction. Thus, the developed probing system might be a potential platform for the effective measurement of single biomolecular level interactions.
机译:提出并开发了一种新的纳米级探测系统,以测量和分析单分子水平上生物分子之间的相互作用力。设计了一种采用原子力显微镜(AFM)工作原理的探测仪器,用于通过图案化的金纳米点阵列和功能化的AFM探针测量单个生物分子的相互作用力。通过测量蛋白质(霍乱弧菌毒素B亚基)和碳水化合物(GM1五糖)之间的解键力来评估构建的探测系统,作为模型单生物分子相互作用。因此,开发的探测系统可能是有效测量单个生物分子水平相互作用的潜在平台。

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