Rapid DNA isolation for marker assisted selection in rice breeding

摘要

During the PCR-based marker assisted pyramiding of disease resistance genes in rice, we developed a DNA isolation protocol suitable for PCR analysis and replaced ethidium bromide by methylene blue to visualise the DNA bands in the gel. The protocol weused for DNA isolation does not require liquid nitrogen, needs only very small amount of tissue sample and is very rapid. Quality of isolated DNA from this protocol is good for PCR analysis. Satisfactory results have been obtained from these DNAs in PCR-based analysis compared to the DNAs extracted by the conventional large scale procedure using liquid nitrogen. These results are reproducible and this protocol is now standard procedure for PCR-based DNA marker-assisted breeding at IRRI.