GENETIC ANALYSIS OF SOMATIC EMBRYO-DERIVED PLANTS OF HEVEA BRASILIENSIS (CLONE RRII105) USING RAPD MARKERS

摘要

Genetic stability of somatic plants developed through somatic embryogenesis from immature anther-derived callus cultures of Hevea brasiliensis (clone RRII 105) was examined using random amplified polymorphic DNA (RAPD) analysis. Ten somatic plants along with their mother tree and 10 monoclonal seedlings were subjected for RAPD analysis and compared. Fifteen arbitrary decamers displayed monomorphic banding profile within all somatic plants and their mother plant. However, polymorphism was detected among monoclonal seedlings with all the five primers tested. UPGMA-based dendrogram showed that all the somatic plants along with the source plant clustered together and no variation was observed among these plants. Absence of polymorphism displayed in the RAPD profile indicated a stable genome in plants developed through somatic embryogenesis.