Cryoradiolytic reduction of heme proteins:Maximizing dose-dependent yield

摘要

Radiolytic reduction in frozen solutions and crystals is a useful method for generation of trapped intermediates in protein-based radical reactions.In this communication we define the conditions which provide the maximum yield of one electron-reduced myoglobin at 77 K using 60Co gamma-irradiation in aqueous glycerol glass.The yield reached 50% after 20kGy,was almost complete at approx 160kGy total dose,and does not depend on the protein concentration in the range 0.01-5 mM.