Relationship between SPAD-502 meter values and extractable chlorophyll in chickpea (Cicer arietinum L.)

摘要

The relationship between SPAD-502 meter values and extractable chlorophyll in chickpea was investigated in experiments conducted at Sultan Qaboos University Agricultural Experiment Station in 2003 and 2004. Chickpea seeds were sown in free-draining plastic pots (22 cm diameter and 30 cm deep) filled with autoclaved sandy loam soil (coarse-silty, mixed, hyperthermic, Torriarents). To induce a range of chlorophyll concentration in the leaves, seedlings were irrigated with a series of nitrate levels ranging from 50 to 150 ppm N two or three times a week. Chlorophyll measurements were made four weeks after seedling emergence with the SPAD-502 meter and by chemical extraction with N, N-dimethylformamide. Extracted chlorophyll contents in the leaves of chickpea were significantly related to SPAD readings in both the 2003 and 2004 experiments. Regression equations of extracted chlorophyll and SPAD-502 meter reading from pooled two years' data produced r~2 values of 0.81 when extracted chlorophyll was expressed on fresh weight basis and 0.84 when expressed on leaf area basis. Based on these results, and in view of the fact that no leaf tissue damage occurred during the SPAD procedure, it was suggested that the SPAD chlorophyll meter could be a useful toolin physiological studies involving chlorophyll determination in chickpea.