Development and validation of rp-hplc method for estimation of levosalbutamol sulphate and ipratropium bromide in pressured meter dose inhaler form

摘要

Simple and sensitive RP-HPLC methods are described for the determination of Levosalbutamol sulphate and Ipratropium bromide combination. The high-performance liquid chromatographic method was developed for the determination of Levosalbutamol sulphate and Ipratropium bromide Pressurized meter dose inhaler (LI-MDI) using acetonitrile: buffer [30: 70] as the mobile phase and measuring the response at max 210 nm. The analysis was performed on a Chromatopak C18 (150 X 4.6 mm), 5-μm column. The method was found to be accurate, with linearity ranging from (80.0% to 120%) for HPLC with a correlation coefficient (r2) 0.999. Precision mean % assay was found with in range.