Isolation of Extreme Halotolerant Bacteria from Asian Desert Dust; Molecular Phytogeny and Growth Properties of their Cells

摘要

We tried the isolation of halophilic bacteria from Asian desert dust falls in Japan and growth property of these bacteria and their molecular phylogeny were analyzed. Two Gram-positive bacteria designated as IMU-1 and IMU-2 were isolated from Asian desert dust. These two strains were adapted with 0-3 and 0-4 M NaCl under nutrient medium culture conditions, respectively, showing the properties of halotolerance. Under the Davis minimal medium culture condition, IMU-1 attained to the similar level of growth as that of nutrient medium culture and growth was observed at 0-2.5 M NaCl. On the other hand, IMU-2 showed the different growth as that of nutrient medium culture condition and growth was observed at 0-1.2 M NaCl. Phylogenetic analysis using 16S rRNA gene sequences revealed that IMU-1 and IMU-2 belong to Bacillus licheniformis and B. megaterium, respectively. It was first study about the isolation of B. licheniformis as the halophilic bacteria in Japan.