Genetic stability assessment of micropropagated mango ginger (Curcuma amada Roxb.) through RAPD and ISSR markers

摘要

Curcuma amada Roxb., popularly known as mango ginger is an important spice and medicinal plant of family Zingiberaceae. In this attempt, amplified polymorphic DNA (RAPD) and Inter Simple Sequence repeats (ISSR) markers were used to determine the genetic stability of micropropagated Curcuma amada. Fifty regenerants were analyzed each at an interval of six months up to two years in culture. Out of 25 RAPD and 10 ISSR primers screened, 19 RAPD primers and 8 ISSR primers gave 3100 and 3300 bands, respectively. RAPD and ISSR analysis revealed monomorphic bands showing the absence of polymorphism in all regenerants analyzed, confirming their genetic uniformity. These results suggest that the micropropagation protocol developed by us for rapid in vitro multiplication is appropriate and applicable for clonal mass propagation of Curcuma amada.