Identification of Genotype and Allelic Frequencies of Vitamin D Receptor Gene (Taqi) Polymorphisms in Type 1 Diabetes Mellitus Patients from Turkey

摘要

Changes in the DNA or polymorphisms of the VDR cause the protein to bifid more or less tightly to 1,250H. The tighter the vitamin D binds, the stronger and longer lasting the metabolic changes are. Some of the different polymorphisms of the VDR have been associated with an increased risk for Diabetes Mellitus. Recent studies suggest that allelic variations of the vitamin D receptor (VDR) gene can influence Diabetes Mellitus. In brief, the aim of this study was to assess the contribution of these VDRpolymorphisms to the susceptibility to Type 1 Diabetes Mellitus in the Turk population. There are several polymorphisms for the VDR gene, but only the three most commonly studied polymorphisms are Taql, Bsml and Fokl examined. This study suggests that while Taql polymorphisms may be functionally different, it may also play a role in serum levels. TT allele of VDR gene has been associated with higher Diabetes Mellitus risk for study on young adults or 100 patients with Type 1 Diabetes Mellitus (DM) (50 women, 50 men) and 120 healthy subjects. The Polymerase chain reaction (PCR) was used for amplification of a 200 bp fragment of the Vitamin D Receptor gene. One study found that TT genotype are overrepresented in Type 1 Diabetes Mellitus patients and those with the TT allele had a 3 fold increase in Type 1 Diabetes Mellitus risk. In addition, the aim of the present study was to adapt PCR amplification, the PCR-RFLP and the most effective DNA isolation method. Taql polymorphism indicates susceptibility toType 1 Diabetes Mellitus in the Turk population. The results indicate that the Taq 1 polymorphism in the VDR gene plays a significant role in protection against T1DM.