Thin Layer Chromatographic Analysis of Free Amino Acids in the Haemolymph and DGG of Indoplanorbis exustus (Mollusca: Gastropoda) Naturally Infected with Digenetic Trematodes

摘要

The purpose of this study was to assess changes induced in free amino acids in the digestive gland-gonad complex and haemolymph of Indoplanorbis exustus naturally infected with larval digenes. A preliminary thin layer chromatography, followed by HPTLCanalysis was used to determine the qualitative status of free amino acids in the uninfected and infected snails. Qualitative analysis showed the presence of seven amino acids, namely, histidine, lysine, alanine, serine, valine, leucine and isoleucine inuninfected DGG and haemolymph, whereas, only lysine was not observed in all infected sample. Alanine was absent in snails infected with Cercariae indicae XVII and Cotylophoron cotylophorom, however, valine also depleted in snails infected with C. cotylophorom. Quantitative analysis of total free amino acids in all infected DGG and haemolymph samples depicted significant decrease (p<0.05) in their concentrations thus clearly indicating depletion of amino acids in parasitized snails.