Effect of sodium nitroprusside, a nitric oxide donor, and aminoguanidine, a nitric oxide synthase inhibitor, on in vitro development of buffalo (Bubalus bubalis) embryos.

摘要

Nitric oxide (NO), a free radical molecule, is produced from l-arginine by the action of nitric oxide synthase (NOS), which exists in three isoforms: inducible (iNOS), endothelial (eNOS) and neuronal (nNOS, Clementi et al. 1999). There are several reports on the putative role NO pathway plays in oocyte maturation in mice (Sengoku et al. 2001) and cattle (Viana et al. 2007). However, the role of NO and iNOS in embryo development is not clear. This study was taken up to investigate the effects of dose-dependent application of sodium nitroprusside (SNP), a NO donor, or aminoguanidine (AG), an inhibitor of iNOS, to in vitro culture (IVC) medium on the development of buffalo (Bubalus bubalis) embryos.