Ion, protein, phospholipid and energy substrate content of oviduct fluid during the oestrous cycle of buffalo (Bubalus bubalis).

摘要

The aim of this research was to analyse the composition of oviduct fluid (ODF) in buffalo cows at different oestrous cycle phases to fulfil the requirements of buffalo embryos in vitro. ODF was collected by chronic cannulation from three cows that were synchronized by administering a synthetic prostaglandin. Based on hormonal profiles, the pre-ovulatory, ovulatory, post-ovulatory and luteal phases of the oestrous cycle were defined. The volume of ODF produced (ml/24 h) was influenced by the oestrous cycle, with values (mean+or-SE) around ovulation (1.0+or-0.2) greater (p<0.05) than in both the luteal (0.4+or-0.1) and the post-ovulatory phases (0.5+or-0.1), but not different from the intermediate values in the pre-ovulatory phase (0.8+or-0.2). Among cycle phases, no differences were found in sodium, potassium, calcium and magnesium concentrations (130.0+or-1.1, 5.1+or-0.3, 2.8+or-0.1 and 0.59+or-0.04 mmol/l respectively). Interestingly, the chloride secretion ( micro M/24 h) was higher (p<0.05) at ovulation (150.2+or-16.5) than during both the luteal (73.7+or-22.0) and the post-ovulatory phases (63.7+or-11.2), with intermediate values in the pre-ovulatory phase (113.4+or-23.5). Glucose concentration (mmol/l) was higher (p=0.056) in the pre-ovulatory phase (0.06+or-0.02) than in the luteal (0.02+or-0.01) and post-ovulatory (0.02+or-0.01) phases but not different from values in the ovulatory phase (0.04+or-0.02). Concentrations of pyruvate and lactate among oestrous cycle phases were similar (0.08+or-0.01 and 1.0+or-0.1 mmol/l respectively). The total quantity of phospholipids ( micro mol/24 h) was greater (p<0.05) at ovulation (0.21+or-0.02) compared with the luteal, pre-ovulatory and post-ovulatory phases of the cycle (0.09+or-0.02, 0.13+or-0.02 and 0.09+or-0.01 respectively). No differences were found in either the protein concentration (1.8+or-0.3 mg/ml) or the quantity of proteins secreted in 24 h (1.8+or-0.4 mg) among oestrous cycle phases. In conclusion, this study provides the first characterization of buffalo ODF during the oestrous cycle, showing species-specific differences that may be useful for developing suitable media for buffalo in vitro embryo production.