Atmospheric pressure infrared (10.6 mu m) laser desorption electrospray ionization (IR-LDESI) coupled to a LTQ Fourier transform ion cyclotron resonance mass spectrometer

摘要

We present the implementation of a CO2 laser emitting infrared laser irradiation at 10.6 mu m onto the versatile atmospheric pressure ionization platform. Infrared laser desorption electrospray ionization (IR-LDESI) is demonstrated from liquid-state samples at atmospheric pressure with and without ESI postionization. Multiply charged proteins ranging in molecular mass from 8.6 to 17kDa were detected from liquid-state samples without the addition of matrix.