Gas chromatography/combustion/isotope-ratio-monitoring mass spectrometric analysis of methylboronic derivatives of monosaccharides: a new method for determining natural C-13 abundances of carbohydrates

摘要

Monosaccharides were derivatized using methylboronic acid and N,O-bis (trimethyisilyl)trifluoroacetamide (BSTFA), and the delta C-13 values of these derivatives measured by gas chromatography/ combustion/isotope-ratio-monitoring mass spectrometry to determine the original C-13-content of the monosaccharides. Comparison with the measured off-line delta (13) values of the monosaccharides shows that no fractionation in C-13 takes place during derivatization. The methylboronic derivatization method has proven to be a new method for natural abundance isotopic analysis of intact monosaccharides (arabinose, xylose, fucose, fructose and glucose). The method is rapid, does not involve isotopic fractionation during derivatization, and gives more precise delta C-13 values than other methods reported. The method was successfully applied to determine the delta C-13 value of glucose of the freshwater alga Scenedesmus communis. Copyright (C) 2001 John Wiley & Sons, Ltd. [References: 10]