A single generic microbore liquid chromatography/time-of-flight mass spectrometry solution for the simultaneous accurate mass determination of compounds on single beads, the decoding of dansylated orthogonal tags pertaining to compounds and accurate

摘要

A single generic microbore liquid chromatography diode array/time-flight mass spectrometry method has been developed that provides accurate mass on separated compounds cleaved from single beads and/or the decoding of orthogonal tags pertaining to the compound in one run. The method is suitable for encoded or non-encoded libraries. Accurate mass measurement within 5ppm can be routinely achieved allowing differentiation of isobaric compounds in degenerate libraries. Accurate mass chromatograms can be used to resolve tag responses from polyethylene glycol background interference. Accurate isotope mass differences coupled with intensity ratios can be used for highly specific isotopic target analysis for compounds of unknown molecular weight but with a characteristic accurate isotope pattern. Accurate instrument calibration is not necessary with this approach to target analysis. Copyright (C) 1999 John Wiley & Sons, Ltd. [References: 11]