Enzyme kinetic characterization of microbe-produced urease for microbe-driven calcite mineralization

摘要

Just as calcite precipitation rate is equal to CO_3~(2-) formation rate, this CO_3~(2-) formation rate is equal to that of urea hydrolysis in microbial calcite precipitation. This formative rate of CO_3~(2-) evolved a mode to assay the urea hydrolysis rate, which was influenced by urea and Ca~(2+) concentrations, pH, and temperature. To contrive the highest V_(max) of the Michaelis-Menten enzyme kinetics equation for the fastest urea hydrolysis rate, 35 °C, pH 10, 2 M urea and 2 M Ca~(2+) were the optimal conditions based on the studies.