Effect of tumor necrosis factor-alpha and interleukin-2 on spleen lymphocyte migration in mouse skin.

摘要

Tumor necrosis factor-alpha (TNF-alpha) and interleukin-2 (IL-2) are reported to enhance lymphocyte binding to endothelial cells in vitro. We examined these two agents on lymphocyte migration in vivo. Spleen lymphocytes were radiolabeled with tritiated uridine (3H-UR) and then injected i.v. into mice. Each cytokine (TNF-alpha or IL-2) or both cytokines were then injected intradermally on the back of mice. The results demonstrated that TNF-alpha stimulates lymphocyte migration in vivo in dose-dependent fashion. Kinetic analysis demonstrated that migration with TNF-alpha started at 3 h, peaked at 6 h, followed by a gradual decline back to baseline at 24 h. IL-2, on the other hand, was nearly inactive, and did not augment lymphocyte migration over and above that induced by TNF-alpha when both cytokines were injected together.