MYBPC1 computational phosphoprotein network construction and analysis between frontal cortex of HIV encephalitis (HIVE) and HIVE-control patients.

摘要

MYBPC1 computational phosphoprotein network construction and analysis of frontal cortex of HIV encephalitis (HIVE) was very useful to identify novel markers and potential targets for prognosis and therapy. Based on integrated gene regulatory network infer method by linear programming and a decomposition procedure with analysis of the significant function cluster using kappa statistics and fuzzy heuristic clustering from the database for annotation, visualization, and integrated discovery, we identified and constructed significant molecule MYBPC1 phosphoprotein network from 12 frontal cortex of HIVEcontrol patients and 16 HIVE in the same GEO Dataset GDS1726. Our result verified MYBPC1 phosphoprotein module only in the upstream of frontal cortex of HIVEcontrol patients (CREB5, MAPKAPK3 inhibition), whereas in the upstream of frontal cortex of HIVE (CREB5, ZC3HAV1 activation; ROR1 inhibition) and downstream (MAPKAPK3 activation; CFDP1, PDCD4, RBBP6 inhibition). Importantly, we determined that MYBPC1 phosphoprotein cluster of HIVE was involved in signal transduction, transferase, post-translational protein modification, developmental process and glycoprotein (only in HIVE terms), the condition was vital to inflammation and cognition impairment of HIVE. Our result demonstrated that common terms in both HIVE-control patients and HIVE included phosphoprotein, organelle, response to stimulus, nucleic acid binding, primary metabolic process, and biological regulation, and these terms were more relative to inflammation and cognition impairment, therefore, we deduced the stronger MYBPC1 phosphoprotein network in HIVE. It would be necessary of the stronger MYBPC1 phosphoprotein function to inflammation and cognition impairment of HIVE.