Partial characterization of lipases produced by a newly isolated Penicillium sp in solid state and submerged fermentation: A comparative study

摘要

The aim of this research was the partial characterization of enzymatic extracts produced by a newly isolated Penicillium sp. in submerged (SmF) and solid state fermentation (SSF). The partial characterization of the crude enzymatic extract obtained by SSF and SmF systems showed optimum activity at pH 5.5 and 47 degrees C, and pH 7.0 and 37 degrees C, respectively (15.17 U/mL and 11.28 U/mL). The crude enzymatic extracts obtained by SmF and SSF presented the best stability at pH from 4.9 to 8.5 and temperature from 25 degrees C to 35 degrees C and pH 7.0 and 25 degrees C, respectively. These results confirm the interesting potential of SSF, because, besides the higher activities obtained in this system, the half-life time at 25 degrees C was higher than that observed for the lipase extract obtained in the SmF system.